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光学相干断层扫描: 成像小鼠视网膜神经节细胞在体内

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo

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光学相干断层扫描: 成像小鼠视网膜神经节细胞在体内

DOI:

10.3791/55865-v

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08:17 min

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September 22, 2017

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Jolanta Jagodzinska*¹, Emmanuelle Sarzi*¹, Mélanie Cavalier, Marie Seveno, Volker Baecker, Christian Hamel^3,4, Marie Péquignot, Cecile Delettre

¹INSERM U1051, Institut of Neurosciences of Montpellier, ²CNRS UMS3426,BioCampus Montepellier, ³University of Montpellier, ⁴CHRU Montpellier, Centre of Reference for Genetic Sensory Diseases,CHU Gui de Chauliac Hospital

Chapters

00:05Title
00:51Pre-imaging Preparation
01:51Mouse Preparation
02:40Mouse Surgical Preparation
04:43Spectral Domain-optical Coherence Tomography (SD-OCT) Optical Nerve Head (ONH) and Retina Imaging
05:31Analysis
06:25Results: Representative Retinal Scanning and Analysis
07:35Conclusion

Summary

Automatic Translation

这篇手稿描述了一个协议为在体内成像的小鼠视网膜与高分辨率光谱领域光学相干断层扫描 (SD OCT)。它的重点是视网膜神经节细胞 (王国) 在 peripapillary 地区, 有几个扫描和量化的方法描述。

Tags

Optical Coherence Tomography OCT Retinal Ganglion Cells Non-invasive Optic Neuropathy Retinal Thickness Mouse Eye Phenylephrine Tropicamide Anesthesia Oxybuprocaine Hydrochloride Glycol Hypromellose Imaging

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