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In Vitro Ensayo de bioluminiscencia para la caracterización del ritmo circadiano en células epiteliales mamarias
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Genetics
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JoVE Journal Genetics
In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
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In Vitro Ensayo de bioluminiscencia para la caracterización del ritmo circadiano en células epiteliales mamarias

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9,558 Views

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11:56 min

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September 28, 2017

DOI:

10.3791/55832-v

DOI:
10.3791/55832-v

•

11:56 min
September 28, 2017

•

7 Views
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1Department of Environmental and Occupational Health, School of Public Health, NIEHS Center for Environmental Exposures and Disease, Environmental and Occupational Health Sciences Institute,Rutgers University, 2Veterinary Drugs & Biologics Division,Animal and Plant Quarantine Agency

Summary

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Se presenta un ensayo de bioluminiscencia en vitro para determinar del ritmo circadiano celular en células epiteliales mamarias. Este método utiliza plásmidos de reportero de células de mamífero expresando desestabilizada luciferasa bajo el control del promotor del gen de periodo 2 . Puede ser adaptado a otros tipos de células para evaluar efectos de órgano-específicas en el ritmo circadiano.

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Fang, M., Kang, H., Park, Y., Estrella, B., Zarbl, H. In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells. J. Vis. Exp. (127), e55832, doi:10.3791/55832 (2017).

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