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In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
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遗传学
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JoVE 杂志 遗传学
In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

9,571 Views

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11:56 min

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September 28, 2017

DOI:

10.3791/55832-v

DOI:
10.3791/55832-v

•

11:56 min
September 28, 2017

•

7 Views
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1Department of Environmental and Occupational Health, School of Public Health, NIEHS Center for Environmental Exposures and Disease, Environmental and Occupational Health Sciences Institute,Rutgers University, 2Veterinary Drugs & Biologics Division,Animal and Plant Quarantine Agency

Summary

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An in vitro bioluminescence assay to determine cellular circadian rhythm in mammary epithelial cells is presented. This method utilizes mammalian cell reporter plasmids expressing destabilized luciferase under the control of the PERIOD 2 gene promoter. It can be adapted to other cell types to evaluate organ-specific effects on circadian rhythm.

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Fang, M., Kang, H., Park, Y., Estrella, B., Zarbl, H. In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells. J. Vis. Exp. (127), e55832, doi:10.3791/55832 (2017).

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