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細胞分化中のマイトジェン活性化プロテインキナーゼ経路の光媒介可逆的調節<em>アフリカツメガエル</em>胚発生
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JoVE Journal Developmental Biology
Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
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細胞分化中のマイトジェン活性化プロテインキナーゼ経路の光媒介可逆的調節<em>アフリカツメガエル</em>胚発生

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8,584 Views

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09:32 min

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June 15, 2017

DOI:

10.3791/55823-v

DOI:
10.3791/55823-v

•

09:32 min
June 15, 2017

•

8 Views
, , , , , , ,
1Department of Biochemistry,University of Illinois at Urbana-Champaign, 2Department of Comparative Biosciences,University of Illinois at Urbana-Champaign, 3Neuroscience Program,University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology,University of Illinois at Urbana-Champaign

Summary

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このプロトコルは、細胞分化およびアフリカツメガエル胚発生の間にマイトジェン活性化タンパク質キナーゼ（MAPK）活性を調節するための光発生戦略を記載する。この方法は、哺乳動物細胞培養物およびアフリカツメガエル胚のような多細胞生きている生物において、高い空間的および時間的分解能を有するMAPKシグナル伝達経路の可逆的活性化を可能にする。

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Krishnamurthy, V. V., Turgeon, A. J., Khamo, J. S., Mondal, P., Sharum, S. R., Mei, W., Yang, J., Zhang, K. Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development. J. Vis. Exp. (124), e55823, doi:10.3791/55823 (2017).

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