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Lichtvermittelte reversible Modulation des mitogenaktivierten Proteinkinase-Pathways während der Zelldifferenzierung und<em> Xenopus</em> Embryonale Entwicklung
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Developmental Biology
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JoVE Journal Developmental Biology
Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
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Lichtvermittelte reversible Modulation des mitogenaktivierten Proteinkinase-Pathways während der Zelldifferenzierung und<em> Xenopus</em> Embryonale Entwicklung

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8,584 Views

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09:32 min

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June 15, 2017

DOI:

10.3791/55823-v

DOI:
10.3791/55823-v

•

09:32 min
June 15, 2017

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8 Views
, , , , , , ,
1Department of Biochemistry,University of Illinois at Urbana-Champaign, 2Department of Comparative Biosciences,University of Illinois at Urbana-Champaign, 3Neuroscience Program,University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology,University of Illinois at Urbana-Champaign

Summary

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Dieses Protokoll beschreibt eine optogenetische Strategie zur Modulation der mitogenaktivierten Proteinkinase (MAPK) Aktivität während der Zelldifferenzierung und der Xenopus embryonalen Entwicklung. Diese Methode ermöglicht die reversible Aktivierung des MAPK-Signalwegs in der Säugerzellkultur und in multizellulären Lebendorganismen wie Xenopus- Embryonen mit hoher räumlicher und zeitlicher Auflösung.

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Krishnamurthy, V. V., Turgeon, A. J., Khamo, J. S., Mondal, P., Sharum, S. R., Mei, W., Yang, J., Zhang, K. Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development. J. Vis. Exp. (124), e55823, doi:10.3791/55823 (2017).

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