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在体外人骨髓间充质干细胞分化为心肌样细胞功能
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Developmental Biology
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JoVE Journal Developmental Biology
In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
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在体外人骨髓间充质干细胞分化为心肌样细胞功能

DOI:
10.3791/55757-v

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09:05 min

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August 09, 2017

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, , ,
1Create Fertility Centre, 2Department of Physiology,University of Toronto, 3Department of Obstetrics and Gynecology,University of Toronto, 4Department of Physiology,University of Toronto, 5Department of Obstetrics and Gynecology,Women’s College Hospital

Chapters

  • 00:05Title
  • 00:58Preparation of Primary Rat Cardiac, Human MSC Co-cultures
  • 03:55Preparation of Aggregate Co-cultures
  • 05:43Results: Differentiation of Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
  • 07:53Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们提出一种方法，有效地利用的心肌细胞分化潜力，年轻的人骨髓间充质干细胞的来源以生成功能、 订约、 心肌样细胞体外。

Tags

Mesenchymal Stem CellsCardiomyocyte DifferentiationIn Vitro DifferentiationCardiac Fetal LayersCardiovascular DiseaseTrypsin DigestionDMEM-F12MSC CulturesCell Dissociation

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