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闪存和冻结:一种新的技术来捕获膜动力学与电子显微镜
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
闪存和冻结:一种新的技术来捕获膜动力学与电子显微镜
DOI:
10.3791/55664-v
•
10:01 min
•
May 01, 2017
•
Shuo Li*
1,2
,
Sumana Raychaudhuri*
1
,
Shigeki Watanabe
3
1
Department of Cell Biology
,
Johns Hopkins School of Medicine
,
2
Department of Biochemistry and Molecular Biology
,
Johns Hopkins Bloomberg School of Public Health
,
3
Solomon H. Snyder Department of Neuroscience
,
Johns Hopkins School of Medicine
Chapters
00:05
Title
00:52
Flash-and-Freeze
03:41
Automated Freeze-substitution Unit-transfer
06:40
Sample Mounting and Imaging
08:24
Results: Representative Exocytosis and Endocytosis in Mouse Hippocampal Neurons
08:56
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们开发的电子显微镜,“闪光和冻结,”一种新的技术,使膜动力学与毫秒的时间分辨率的可视化。该技术结合了高压冷冻神经元的光遗传学刺激。这里,我们证明的程序和详细描述了协议。
Tags
Electron Microscopy
Membrane Dynamics
Optogenetics
Synaptic Vesicle Recycling
Flash-and-freeze
High-pressure Freezing
Free Substitution
Time-resolved Imaging
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