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Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy
JoVE 杂志
生物学
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JoVE 杂志 生物学
Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

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10:01 min

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May 01, 2017

DOI:

10.3791/55664-v

DOI:
10.3791/55664-v

•

10:01 min
May 01, 2017

•

1 Views
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1Department of Cell Biology,Johns Hopkins School of Medicine, 2Department of Biochemistry and Molecular Biology,Johns Hopkins Bloomberg School of Public Health, 3Solomon H. Snyder Department of Neuroscience,Johns Hopkins School of Medicine

Tags

Electron MicroscopyMembrane DynamicsOptogeneticsSynaptic Vesicle RecyclingFlash-and-freezeHigh-pressure FreezingFree SubstitutionTime-resolved Imaging

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Li, S., Raychaudhuri, S., Watanabe, S. Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy. J. Vis. Exp. (123), e55664, doi:10.3791/55664 (2017).

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