ジャーナル
/
生化学
/
Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
JoVE Journal
生化学
This content is Free Access.
JoVE Journal 生化学
Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

20,130 Views

•

15:27 min

•

April 17, 2017

DOI:

10.3791/55659-v

DOI:
10.3791/55659-v

•

15:27 min
April 17, 2017

•

4 Views
, , ,
1Department of Biotherapeutics Discovery, Immune Modulation and Biotherapeutics Discovery,Boehringer Ingelheim Pharmaceuticals, Inc., 2The Fu Foundation School of Engineering and Applied Science,Columbia University

概要

Automatically generated

We describe here protocols for the measurement of antibody-antigen binding affinity and kinetics using four commonly used biosensor platforms.

Explore More Videos

Antibody-antigen BindingBiosensor PlatformsKinetic DataDrug DiscoveryBiacore T100ProteOn XPR36Experimental DesignKinetic MeasurementsHigh-affinity InteractionsData ConsistencyOperational Efficiency

Read Article

Cite this Article

Yang, D., Singh, A., Wu, H., Kroe-Barrett, R. Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms. J. Vis. Exp. (122), e55659, doi:10.3791/55659 (2017).

Download .ris file

Copy

Share Video

.

Copy