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斑马鱼急性酒精性肝损伤的组织学分析

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Developmental Biology

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JoVE Journal Developmental Biology

Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish

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斑马鱼急性酒精性肝损伤的组织学分析

DOI:

10.3791/55630-v

•

10:45 min

•

May 25, 2017

•

Jillian L. Ellis, Chunyue Yin²

¹Division of Gastroenterology, Hepatology and Nutrition,Cincinnati Children’s Hospital Medical Center, ²Division of Developmental Biology,Cincinnati Children’s Hospital Medical Center

Chapters

00:05Title
00:50Acute Ethanol Treatment in Zebrafish Larvae
01:57Preparation of Tissue Cassettes and Processing
04:02Tissue Sectioning
05:49Hematoxylin and Eosin (H&E) Staining of Paraffin-embedded Sections
08:44Results: Comparisons of H&E Staining in the Livers of Zebrafish Larvae
10:14Conclusion

Summary

Automatic Translation

该方案描述了用2％乙醇处理24小时的斑马鱼幼虫肝脏的组织学分析。这种急性乙醇治疗导致肝血管的肝脂肪变性和肿胀。

Tags

Zebrafish Histology Acute Alcoholic Liver Injury Patho-histology Ethanol Alcohol Liver Disease 2% Ethanol Dietrich’s Fixative PBS 3% Agarose Sagittal Sections

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