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Structuur-functie studies in muis embryonale stamcellen gebruiken recombinase-gemedieerde cassette uitwisseling
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Developmental Biology
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JoVE Journal Developmental Biology
Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange
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Structuur-functie studies in muis embryonale stamcellen gebruiken recombinase-gemedieerde cassette uitwisseling

Nederlands

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10,770 Views

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15:13 min

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April 27, 2017

DOI:

10.3791/55575-v

DOI:
10.3791/55575-v

•

15:13 min
April 27, 2017

•

13 Views
, , , , , , , , , ,
1Department of Biomedical Molecular Biology,Ghent University, 2Inflammation Research Center,VIB, 3Center for Medical Genetics,Ghent University Hospital, 4Cancer Research Institute Ghent (CRIG), 5Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences,Ghent University, 6Helmholtz Center for Infection Research, 7Mammalian Functional Genetics Laboratory, Division of Blood Cancers, Australian Centre for Blood Diseases, Department of Clinical Haematology,Monash University and Alfred Health Alfred Centre

Summary

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Eiwitten vaak meerdere domeinen die verschillende cellulaire functies kunnen uitoefenen. Gene knock-outs (KO) beschouw dit niet als functionele diversiteit. Hier melden wij een recombinatie-gemedieerde cassette uitwisseling (RMCE) gebaseerde structuur-functie benadering KO embryonale stamcellen waarmee de moleculaire dissectie van verschillende functionele domeinen of varianten van een eiwit.

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Cite this Article

Pieters, T., Haenebalcke, L., Bruneel, K., Vandamme, N., Hochepied, T., van Hengel, J., Wirth, D., Berx, G., Haigh, J. J., van Roy, F., Goossens, S. Structure-function Studies in Mouse Embryonic Stem Cells Using Recombinase-mediated Cassette Exchange. J. Vis. Exp. (122), e55575, doi:10.3791/55575 (2017).

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