Journal
/
Biochemistry
/
التعبير، تنقية، ومضادات الميكروبات النشاط من S100A12
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Expression, Purification, and Antimicrobial Activity of S100A12
Please note that all translations are automatically generated. Click here for the English version.

التعبير، تنقية، ومضادات الميكروبات النشاط من S100A12

العربية

Automatically Generated

8,196 Views

•

11:10 min

•

May 13, 2017

DOI:

10.3791/55557-v

DOI:
10.3791/55557-v

•

11:10 min
May 13, 2017

•

9 Views
, , , ,
1Department of Life and Physical Sciences,Fisk University, 2Tennessee Valley Healthcare Systems,U. S. Dept. of Veterans Affairs, 3Department of Medicine – Division of Infectious Diseases,Vanderbilt University Medical School, 4Department of Biochemistry,Vanderbilt University

Summary

Automatically generated

هنا، نقدم طريقة للتعبير عن وتنقية S100A12 (كالغرانولين C). نحن تصف بروتوكول لقياس نشاطها المضادة للميكروبات ضد الممرض البشري H. بيلوري .

Explore More Videos

S100A12Antimicrobial PeptideProtein ExpressionProtein PurificationAntimicrobial ActivityMetal SequestrationOligomerizationCalprotectinBL21 DE3 CellsAutoinduction MediaCentrifugationSonicationAffinity ChromatographySize Exclusion Chromatography

Read Article

Cite this Article

Jackson, E., Little, S., Franklin, D. S., Gaddy, J. A., Damo, S. M. Expression, Purification, and Antimicrobial Activity of S100A12. J. Vis. Exp. (123), e55557, doi:10.3791/55557 (2017).

Download .ris file

Copy

Share Video

.

Copy