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Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

9,979 Views

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11:37 min

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July 03, 2017

DOI:

10.3791/55536-v

DOI:
10.3791/55536-v

•

11:37 min
July 03, 2017

•

5 Views
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1Unité de Pathogénie Microbienne Moléculaire, INSERM U786,Institut Pasteur, 2Microbiologie et Maladies Infectieuses,Collège de France, 3Laboratory of Intestinal Immunity, Institut Imagine-INSERM UMR 1163,Université Paris Descartes-Sorbonne Paris Cité

Summary

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We describe a method for the qualitative and quantitative analysis of stress granule formation in mammalian cells after the cells are challenged with bacteria and a number of different stresses. This protocol can be applied to investigate the cellular stress granule response in a wide range of host-bacterial interactions.

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Cite this Article

Vonaesch, P., Sansonetti, P. J., Schnupf, P. Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells. J. Vis. Exp. (125), e55536, doi:10.3791/55536 (2017).

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