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Protocollen voor het visualiseren steroidogenic organen en hun Interactive Organen met Immunokleuring in de fruitvlieg<em> Drosophila melanogaster</em
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Developmental Biology
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JoVE Journal Developmental Biology
Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster
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Protocollen voor het visualiseren steroidogenic organen en hun Interactive Organen met Immunokleuring in de fruitvlieg<em> Drosophila melanogaster</em

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10,245 Views

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12:10 min

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April 14, 2017

DOI:

10.3791/55519-v

DOI:
10.3791/55519-v

•

12:10 min
April 14, 2017

•

1 Views
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1Graduate School of Life and Environmental Sciences,University of Tsukuba, 2Life Science Center of Tsukuba Advanced Research Alliance,University of Tsukuba, 3Faculty of Life and Environmental Sciences,University of Tsukuba

Summary

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We beschrijven een protocol voor dissectie, fixatie en immunokleuring van steroidogenic organen in Drosophila larven en volwassen vrouwtjes steroïde hormonen biosynthese en haar reguleringsmechanisme bestuderen. Naast steroïdogene organen visualiseren we de innervatie van steroidogenic organen en steroidogene doelcellen zoals kiemlijn stamcellen.

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Imura, E., Yoshinari, Y., Shimada-Niwa, Y., Niwa, R. Protocols for Visualizing Steroidogenic Organs and Their Interactive Organs with Immunostaining in the Fruit Fly Drosophila melanogaster. J. Vis. Exp. (122), e55519, doi:10.3791/55519 (2017).

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