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マウス胚線維芽細胞の転写因子媒介リプログラミングに続いて心筋細胞のサブタイプを評価します
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts
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マウス胚線維芽細胞の転写因子媒介リプログラミングに続いて心筋細胞のサブタイプを評価します

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7,309 Views

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09:29 min

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March 22, 2017

DOI:

10.3791/55456-v

DOI:
10.3791/55456-v

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09:29 min
March 22, 2017

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3 Views
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1Department of Internal Medicine- Cardiology,UT Southwestern Medical Center

Summary

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This manuscript describes a step-by-step protocol for the generation and quantification of diverse reprogrammed cardiac subtypes using a retrovirus-mediated delivery of Gata4, Hand2, Mef2c, and Tbx5.

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Fernandez-Perez, A., Munshi, N. V. Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts. J. Vis. Exp. (121), e55456, doi:10.3791/55456 (2017).

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