/
/
Un protocolo para la rápida post-mortem de cultivos celulares de glioma pontino intrínsecos difusos (DIPG)
This content is Free Access.
1Graduate Program in Neuroscience, Department of Neurology, Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine, 2Departments of Neurology, Neurosurgery, Pathology and Pediatrics, Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine
Chapters
- 00:05Title
- 01:12Mechanical Dissociation
- 03:25Enzymatic Dissociation
- 04:50Sucrose Gradient Centrifugation
- 05:49ACK Red Blood Cell Lysis and Initial Culture Maintenance
- 06:50Results: Images of Primary Cultures and Early-passage Cells
- 07:41Conclusion
Este protocolo describe un método para el rápido procesamiento de las muestras difusas pontina glioma intrínseco post mortem para la creación de modelos de cultivos celulares derivados de pacientes o la caracterización directa de las células tumorales y microambientales.
