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Um protocolo para a Cultura rápidos post-mortem celular de Difuso Intrínseca Pontine glioma (DIPG)
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March 07, 2017
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1Graduate Program in Neuroscience, Department of Neurology, Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine, 2Departments of Neurology, Neurosurgery, Pathology and Pediatrics, Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine
Summary
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Este protocolo descreve um método para o rápido processamento de amostras de glioma pontino intrínsecas difusas post-mortem para o estabelecimento de modelos de culturas de células derivadas de pacientes ou caracterização directa de células tumorais e microambiente.
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Lin, G. L., Monje, M. A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma (DIPG). J. Vis. Exp. (121), e55360, doi:10.3791/55360 (2017).
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