Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells

Urszula Grabiec; Tim Hohmann; Niels Hammer; Faramarz Dehghani

doi:10.3791/55359

Journal

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Neuroscience

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Organotypic hippocampus skive kulturer som en Model til undersøgelse Neuroprotection og invasionsevne af tumorceller

JoVE Journal
Neuroscience
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JoVE Journal Neuroscience

Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells

Organotypic hippocampus skive kulturer som en Model til undersøgelse Neuroprotection og invasionsevne af tumorceller

DOI:

10.3791/55359-v

•

07:48 min

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August 27, 2017

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Urszula Grabiec*¹, Tim Hohmann*¹, Niels Hammer, Faramarz Dehghani

¹Department of Anatomy and Cell Biology,Martin Luther University Halle-Wittenberg, ²Department of Anatomy,University of Otago

Chapters

00:00Title
00:08Agar Preparation
00:54Brain Fixation
02:29Slicing Procedure
03:26Hippocampus Removal
04:05Culturing Method
05:18Representative Results
07:19Conclusion

Summary

Automatic Translation

Organotypic hippocampus skive kulturer (OHSC) repræsenterer en in vitro- model, der simulerer i vivo situationen meget godt. Her beskriver vi en vibratome-baseret forbedret udskæring protokol for at opnå høj kvalitet skiver til brug ved vurderingen af neuroprotektive potentiale af nye stoffer eller den biologiske opførsel af tumorceller.

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The Organotypic Hippocampal Slice Culture Model for Examining Neuronal Injury