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Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells

Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells

12,575 Views

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07:48 min

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August 27, 2017

DOI:

10.3791/55359-v

DOI:
10.3791/55359-v

•

07:48 min
August 27, 2017

•

5 Views
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1Department of Anatomy and Cell Biology,Martin Luther University Halle-Wittenberg, 2Department of Anatomy,University of Otago

Summary

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Organotypic hippocampal slice cultures (OHSC) represent an in vitro model that simulates the in vivo situation very well. Here we describe a vibratome-based improved slicing protocol to obtain high quality slices for use in assessing the neuroprotective potential of novel substances or the biological behavior of tumor cells.

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Grabiec, U., Hohmann, T., Hammer, N., Dehghani, F. Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells. J. Vis. Exp. (126), e55359, doi:10.3791/55359 (2017).

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