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Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method
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Neuroscience
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JoVE Journal Neuroscience
Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method

Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method

English

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17,746 Views

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10:26 min

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April 18, 2017

DOI:

10.3791/55358-v

DOI:
10.3791/55358-v

•

10:26 min
April 18, 2017

•

16 Views
, , , ,
1Department of Biomedical Sciences, Ontario Veterinary College,University of Guelph

Summary

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We present a protocol for using the Golgi-Cox staining method in thick brain sections, in order to visualize neurons with long dendritic trees contained within single tissue samples. Two variants of this protocol are also presented that involve cresyl violet counterstaining, and the freezing of unprocessed brains for long-term storage.

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Cite this Article

Louth, E. L., Sutton, C. D., Mendell, A. L., MacLusky, N. J., Bailey, C. D. Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method. J. Vis. Exp. (122), e55358, doi:10.3791/55358 (2017).

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