Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method
17,731 Views
•
10:26 min
•
April 18, 2017
DOI:
Özet
Automatically generated
We present a protocol for using the Golgi-Cox staining method in thick brain sections, in order to visualize neurons with long dendritic trees contained within single tissue samples. Two variants of this protocol are also presented that involve cresyl violet counterstaining, and the freezing of unprocessed brains for long-term storage.
Read Article
Cite this Article
Louth, E. L., Sutton, C. D., Mendell, A. L., MacLusky, N. J., Bailey, C. D. Imaging Neurons within Thick Brain Sections Using the Golgi-Cox Method. J. Vis. Exp. (122), e55358, doi:10.3791/55358 (2017).
Copy