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多量-PAGE：生体試料中のキャプチャと解決タンパク質複合体のための方法
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Biochemistry
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JoVE Journal Biochemistry
Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples
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多量-PAGE：生体試料中のキャプチャと解決タンパク質複合体のための方法

日本語

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11,120 Views

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07:40 min

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May 05, 2017

DOI:

10.3791/55341-v

DOI:
10.3791/55341-v

•

07:40 min
May 05, 2017

•

1 Views
, , ,
1Physiology,Michigan State University, 2Center for Neurodegenerative Science Van Andel Institute, 3Pharmaceutical Sciences,Wayne State University

Summary

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新規二次元ポリアクリルアミドゲル電気泳動（PAGE）システムが提示されるに結合アミン反応性タンパク質架橋剤を用いて修飾されていない組織溶解物から天然タンパク質複合体を安定化し、分離するための方法。

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Rhinesmith, T., Killinger, B. A., Sharma, A., Moszczynska, A. Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples. J. Vis. Exp. (123), e55341, doi:10.3791/55341 (2017).

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