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Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples
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Biochemistry
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JoVE Journal Biochemistry
Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples

Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples

English

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11,129 Views

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07:40 min

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May 05, 2017

DOI:

10.3791/55341-v

DOI:
10.3791/55341-v

•

07:40 min
May 05, 2017

•

1 Views
, , ,
1Physiology,Michigan State University, 2Center for Neurodegenerative Science Van Andel Institute, 3Pharmaceutical Sciences,Wayne State University

Summary

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A method for stabilizing and separating native protein complexes from unmodified tissue lysate using an amine-reactive protein cross-linker coupled to a novel two-dimensional polyacrylamide gel electrophoresis (PAGE) system is presented.

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Cite this Article

Rhinesmith, T., Killinger, B. A., Sharma, A., Moszczynska, A. Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples. J. Vis. Exp. (123), e55341, doi:10.3791/55341 (2017).

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