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Muizen-lymfocyt Labeling door<sup> 64</sup> Cu-Antibody Receptor targeting voor<em> In Vivo</em> Celverkeer PET / CT
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Immunology and Infection
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JoVE Journal Immunology and Infection
Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
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Muizen-lymfocyt Labeling door<sup> 64</sup> Cu-Antibody Receptor targeting voor<em> In Vivo</em> Celverkeer PET / CT

Nederlands

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8,634 Views

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11:34 min

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April 29, 2017

DOI:

10.3791/55270-v

DOI:
10.3791/55270-v

•

11:34 min
April 29, 2017

•

2 Views
, , , , , ,
1Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy,Eberhard Karls University Tübingen, 2Department of Dermatology,Eberhard Karls University Tübingen

Summary

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Na de bereiding van een 64 Cu-gemodificeerd monoklonaal antilichaam dat aan een transgene muizen T-celreceptor, T-cellen radiogelabeld in vivo geanalyseerd op levensvatbaarheid functionaliteit labeling stabiliteit en apoptose en adoptief overgebracht in muizen met een luchtweg vertraagde hypersensitiviteit reactie voor niet-invasieve beeldvorming met positron emission tomography / computed tomography (PET / CT).

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Cite this Article

Hoffmann, S. H. L., Maurer, A., Reck, D. I., Reischl, G., Pichler, B. J., Kneilling, M., Griessinger, C. M. Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT. J. Vis. Exp. (122), e55270, doi:10.3791/55270 (2017).

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