JoVE Journal
Biochemistry
Biochemistry
需要订阅 JoVE 才能查看此 内容. 登录或开始免费试用。
章节
总结概括
This protocol describes the production of a therapeutic antibody in a mammalian expression system. The methods described include preparation of vector DNA, stable transfection and serum-free adaptation of a human embryonic kidney 293 cell line, set up of large scale cultures and purification using affinity chromatography.