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In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity
Chapters
- 00:05Title
- 00:48Injection of DNA and Electroporation
- 01:52Sectioning and Staining of Brain Tissue
- 04:17Imaging and Analysis
- 05:42Electrophysiology
- 08:29Results: Representative Firing Pattern of a CAG-GFP Electroporated Pyramidal Neuron
- 09:39Conclusion
This manuscript provides protocols that use in utero electroporation (IUE) to describe the structural connectivity of neurons at the single-cell level and the excitability of fluorescently labeled neurons. Histology is used to characterize dendritic and axonal projections. Whole-cell recording in acute slices is used to investigate excitability.
