/
/
Quantificazione dei batteri istidina chinasi autophosphorylation usando un test nitrocellulosa Binding
A subscription to JoVE is required to view this content. Sign in or start your free trial.
8,408 Views
•
09:17 min
•
January 11, 2017
DOI:
Summary
Automatically generated
We report and demonstrate an optimized nitrocellulose binding assay that can be used to quantify autophosphorylation of purified bacterial histidine kinases. Our method has several advantages over traditional SDS-PAGE based techniques, providing a valuable alternative for characterizing these important proteins.
Explore More Videos
Read Article
Cite this Article
Fischer, J., Johnson, R. A., Boon, E. Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay. J. Vis. Exp. (119), e55129, doi:10.3791/55129 (2017).
Copy