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Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
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Biochemie
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JoVE Journal Biochemie
Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

8,408 Views

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09:17 min

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January 11, 2017

DOI:

10.3791/55129-v

DOI:
10.3791/55129-v

•

09:17 min
January 11, 2017

•

1 Views
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1Department of Chemistry,Stony Brook University, 2Department of Physiology and Biophysics,Stony Brook University

Summary

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We report and demonstrate an optimized nitrocellulose binding assay that can be used to quantify autophosphorylation of purified bacterial histidine kinases. Our method has several advantages over traditional SDS-PAGE based techniques, providing a valuable alternative for characterizing these important proteins.

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Fischer, J., Johnson, R. A., Boon, E. Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay. J. Vis. Exp. (119), e55129, doi:10.3791/55129 (2017).

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