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الشعرية الكهربائي فصل حيدة النسيلة الأجسام المضادة الإسوية باستخدام الشعرية محايد
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
DOI:

14:53 min

January 16, 2017

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Chapters

  • 00:00Title
  • 00:56Preparation of Background Electrolyte (BGE) Solution
  • 02:18Sample Preparation
  • 03:45CE System Cleaning
  • 05:46Cartridge Assembly
  • 09:41Preparation of Buffer Trays
  • 10:44CE System Components Assembly
  • 12:10Instrument Methods Set-up
  • 13:21Data Acquisition and Processing
  • 14:11Conclusion

Summary

Automatic Translation

Here, we present a comprehensive capillary zone electrophoresis protocol for the assessment of intrinsic physicochemical heterogeneity of monoclonal antibodies as a quality attribute.

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