Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

Carlos Eduardo Espinosa-de la Garza; Rodolfo Daniel Salazar-Flores; N&#233;stor Octavio P&#233;rez; Luis Francisco Flores-Ortiz; Emilio Medina-Rivero

doi:10.3791/55082

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Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

JoVE Journal
Biyokimya
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JoVE Journal Biyokimya

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

12,457 Views

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14:53 min

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January 16, 2017

DOI:

10.3791/55082-v

DOI:

10.3791/55082-v

•

14:53 min

January 16, 2017

•

3 Views

Carlos Eduardo Espinosa-de la Garza*¹, Rodolfo Daniel Salazar-Flores*¹, Néstor Octavio Pérez¹, Luis Francisco Flores-Ortiz¹, Emilio Medina-Rivero¹

¹Unidad de Investigación y Desarrollo, Probiomed S.A. de C.V.

Özet

Here, we present a comprehensive capillary zone electrophoresis protocol for the assessment of intrinsic physicochemical heterogeneity of monoclonal antibodies as a quality attribute.

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Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

Özet

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