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Preparação e cultivo de células precursoras Miogênica / mioblastos primárias de músculo esquelético de Adulto e Idoso Humanos
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans

Preparação e cultivo de células precursoras Miogênica / mioblastos primárias de músculo esquelético de Adulto e Idoso Humanos

21,312 Views

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10:10 min

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February 16, 2017

DOI:

10.3791/55047-v

DOI:
10.3791/55047-v

•

10:10 min
February 16, 2017

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7 Views
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1Institute of Ageing and Chronic Disease,University of Liverpool, 2Aintree University Hospital

Tags

Myogenic Precursor CellsPrimary MyoblastsSkeletal MuscleAdultAgedIsolationIn Vitro ModelMuscle HomeostasisAgingLamininEnzymatic DigestionDMEMDPBSEthanolGrowth MediaCell StrainerCentrifugation

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Soriano-Arroquia, A., Clegg, P. D., Molloy, A. P., Goljanek-Whysall, K. Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans. J. Vis. Exp. (120), e55047, doi:10.3791/55047 (2017).

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