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Developmental Biology

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JoVE Journal Developmental Biology
Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
 

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans

Article DOI: 10.3791/55047-v • 10:10 min • February 16th, 2017
February 16th, 2017 •
1, 1, 1,2, 1
1Institute of Ageing and Chronic Disease, University of Liverpool, 2Aintree University Hospital

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This protocol describes a robust, reproducible and simple method of isolation and culture of myoblast progenitor cells from the skeletal muscle of adult and aged people. The muscles used here include foot and leg muscles. This approach enables the isolation of an enriched population of primary myoblasts for functional studies.

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Preparation Culture Myogenic Precursor Cells Primary Myoblasts Skeletal Muscle Adult Humans Aged Humans Isolation In Vitro Model Muscle Homeostasis Aging Stressor Centrifugation Long Time Culture Phenotype Petri Dish Laminin Solution Incubation Enzymatic Solution Serum Free DMEM Filter Membrane Sterilization Sample Collection Digestion Muscle Biopsy
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