/
/
Производство, Кристаллизация и структура Определение<em> C. несговорчивый</em> PPEP-1 с помощью Microseeding и цинк-SAD
A subscription to JoVE is required to view this content. Sign in or start your free trial.
11,373 Views
•
13:34 min
•
December 30, 2016
DOI:
Summary
Automatically generated
Proline-proline endopeptidase-1 (PPEP-1) is a secreted metalloprotease and promising drug-target from the human pathogen Clostridium difficile. Here we describe all methods necessary for the production and structure determination of this protein.
Read Article
Cite this Article
Pichlo, C., Montada, A. A., Schacherl, M., Baumann, U. Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD. J. Vis. Exp. (118), e55022, doi:10.3791/55022 (2016).
Copy