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Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD
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JoVE Journal Biyokimya
Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD

11,373 Views

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13:34 min

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December 30, 2016

DOI:

10.3791/55022-v

DOI:
10.3791/55022-v

•

13:34 min
December 30, 2016

•

5 Views
, , ,
1Institute of Biochemistry,University of Cologne

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Proline-proline endopeptidase-1 (PPEP-1) is a secreted metalloprotease and promising drug-target from the human pathogen Clostridium difficile. Here we describe all methods necessary for the production and structure determination of this protein.

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CrystallizationStructure DeterminationC. Difficile PPEP-1MicroseedingZinc-SADProtein PurificationProtein ConcentrationSitting DropCrystallization ScreeningCrystal GrowthProtein Structure

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Pichlo, C., Montada, A. A., Schacherl, M., Baumann, U. Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD. J. Vis. Exp. (118), e55022, doi:10.3791/55022 (2016).

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