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Utilizzando impalcatura liposomi per ricostituire interazioni proteina-proteina lipidi-prossimale<em> In Vitro</em

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

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Utilizzando impalcatura liposomi per ricostituire interazioni proteina-proteina lipidi-prossimale<em> In Vitro</em

8,563 Views

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08:53 min

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January 11, 2017

DOI:

10.3791/54971-v

DOI:

10.3791/54971-v

•

08:53 min

January 11, 2017

•

2 Views

Ryan W. Clinton, Jason A. Mears

¹Department of Pharmacology, Center for Mitochondrial Diseases, The Cleveland Center for Membrane and Structural Biology,Case Western Reserve University School of Medicine

Tags

Scaffold Liposomes Lipid-proximal Protein-protein Interactions Mitochondrial Dynamics Protein Complexes Membrane Compartments Lipid Templates Native Confirmation Lipid Cofactors Mitochondrial Fission Machinery His-tagged Proteins Mff Buffer A BME Lipid Extrusion

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