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Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
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JoVE Journal Biyokimya
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

8,594 Views

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08:53 min

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January 11, 2017

DOI:

10.3791/54971-v

DOI:
10.3791/54971-v

•

08:53 min
January 11, 2017

•

6 Views
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1Department of Pharmacology, Center for Mitochondrial Diseases, The Cleveland Center for Membrane and Structural Biology,Case Western Reserve University School of Medicine

Özet

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This paper describes a method for assessing the interactions and assemblies of integral membrane proteins in vitro with various partner factors in a lipid-proximal environment.

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Clinton, R. W., Mears, J. A. Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro. J. Vis. Exp. (119), e54971, doi:10.3791/54971 (2017).

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