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Da un prodotto naturale al suo grappolo Biosynthetic Gene: Una dimostrazione che utilizzino Polyketomycin da<em> Streptomyces diastatochromogenes</em> Tü6028
JoVE Journal
Genetics
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JoVE Journal Genetics
From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
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Da un prodotto naturale al suo grappolo Biosynthetic Gene: Una dimostrazione che utilizzino Polyketomycin da<em> Streptomyces diastatochromogenes</em> Tü6028

italiano

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16,782 Views

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09:08 min

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January 13, 2017

DOI:

10.3791/54952-v

DOI:
10.3791/54952-v

•

09:08 min
January 13, 2017

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12 Views
, , ,
1Department of Pharmaceutical Biology and Biotechnology,Albert-Ludwigs-Universität Freiburg, Germany, 2Department of Chemistry and Biology,Universität Siegen

Summary

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Presentiamo qui un protocollo dettagliato di (A) l'identificazione di un prodotto naturale con attività antibiotica, (B) la purificazione del composto (C) il primo modello della sua biosintesi, (D) sequenziamento del genoma / -mining e ( E) la verifica del cluster genico biosintetico.

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Cite this Article

Greule, A., Zhang, S., Paululat, T., Bechthold, A. From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028. J. Vis. Exp. (119), e54952, doi:10.3791/54952 (2017).

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