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From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
JoVE Journal
Genética
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JoVE Journal Genética
From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

16,757 Views

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09:08 min

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January 13, 2017

DOI:

10.3791/54952-v

DOI:
10.3791/54952-v

•

09:08 min
January 13, 2017

•

12 Views
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1Department of Pharmaceutical Biology and Biotechnology,Albert-Ludwigs-Universität Freiburg, Germany, 2Department of Chemistry and Biology,Universität Siegen

Summary

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Here we present a detailed protocol of (A) the identification of a natural product with antibiotic activity, (B) the purification of the compound, (C) the first model of its biosynthesis, (D) genome sequencing/-mining and the (E) verification of the biosynthetic gene cluster.

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Natural ProductBiosynthetic Gene ClusterPolyketomycinStreptomyces DiastatochromogenesAntibiotic ActivityGenome MiningStreptomycesAntibiotic ProducerBiosynthetic PathwayCompound ExtractionHPLCDisc Diffusion AssayTest StrainAntibiotic

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Cite this Article

Greule, A., Zhang, S., Paululat, T., Bechthold, A. From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028. J. Vis. Exp. (119), e54952, doi:10.3791/54952 (2017).

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