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Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro
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Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro
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January 25, 2017
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We describe here a method for obtaining and identifying a newly characterized subpopulation of neutrophil-derived giant phagocytes. These cells develop in culture from fresh human blood neutrophils, and are characterized by phagocytosis, autophagy, immensely large size, and extended lifespan. This method is essential to further investigate this unique neutrophil-derived subpopulation.
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Lavie, L., Dyugovskaya, L., Polyakov, A., Rogovoy, O., Leder, E. Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro. J. Vis. Exp. (119), e54826, doi:10.3791/54826 (2017).
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