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Generazione rapida ed efficace delle ricombinante cellule staminali umane pluripotenti da ricombinasi mediata scambio Cassette in<em> AAVS1</em> Locus
JoVE Journal
Medicine
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JoVE Journal Medicine
Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus
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Generazione rapida ed efficace delle ricombinante cellule staminali umane pluripotenti da ricombinasi mediata scambio Cassette in<em> AAVS1</em> Locus

italiano

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9,493 Views

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11:36 min

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November 20, 2016

DOI:

10.3791/54718-v

DOI:
10.3791/54718-v

•

11:36 min
November 20, 2016

•

5 Views
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1Stem Cell Institute,Katholieke Universiteit Leuven

Summary

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Qui riportiamo un metodo di modifica genetica rapida ed efficiente in base RMCE nel locus AAVS1 di umani cellule staminali pluripotenti (hPSCs) che migliora i sistemi precedentemente descritti. Usando questa tecnica, linee isogeniche possono essere rapidamente e affidabile generati per adeguati studi comparativi, facilitando la ricerca transgenesi mediata con hPSCs.

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Ordovás, L., Boon, R., Pistoni, M., Chen, Y., Sambathkumar, R., Helsen, N., Vanhove, J., Berckmans, P., Cai, Q., Vanuytsel, K., Raitano, S., Verfaillie, C. M. Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus. J. Vis. Exp. (117), e54718, doi:10.3791/54718 (2016).

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