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Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus

JoVE Revista
Medicina

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JoVE Revista Medicina

Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus

Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus

9,392 Views

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11:36 min

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November 20, 2016

DOI:

10.3791/54718-v

DOI:

10.3791/54718-v

•

11:36 min

November 20, 2016

•

2 Views

Laura Ordovás*¹, Ruben Boon*¹, Mariaelena Pistoni, Yemiao Chen, Rangarajan Sambathkumar, Nicky Helsen, Jolien Vanhove, Pieter Berckmans, Qing Cai, Kim Vanuytsel, Susanna Raitano, Catherine M. Verfaillie

¹Stem Cell Institute,Katholieke Universiteit Leuven

Tags

Recombinant Human Pluripotent Stem Cells AAVS1 Locus Recombinase-mediated Cassette Exchange RMCE Transgenesis Stem Cell Biology Overexpression Knockdown Random Integration Karyotypes IMEFs ROCK Inhibitor Nucleofection Trypsin Accutase Feeder-free

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