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過敏性腸症候群におけるmiRNA循環の摂動は多重化ハイスループット遺伝子発現プラットフォームを使用して検出します
JoVE Journal
Genetics
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JoVE Journal Genetics
Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
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過敏性腸症候群におけるmiRNA循環の摂動は多重化ハイスループット遺伝子発現プラットフォームを使用して検出します

日本語

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8,045 Views

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10:37 min

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November 30, 2016

DOI:

10.3791/54693-v

DOI:
10.3791/54693-v

•

10:37 min
November 30, 2016

•

1 Views
, , , , ,
1Digestive Disorders Unit, National Institute of Nursing Research,National Institutes of Health, DHHS, 2National Institutes of Health Research Scholar,Howard Hughes Medical Institute, 3Internal Medicine, Medical School,University of Michigan

Summary

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We describe the use of a multiplexed high-throughput gene expression platform that quantitates gene expression by barcoding and counting molecules in biological substrates without the need for amplification. We used the platform to quantitate microRNA (miRNA) expression in whole blood in subjects with and without irritable bowel syndrome.

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Cite this Article

Fourie, N. H., Peace, R. M., Abey, S. K., Sherwin, L. B., Wiley, J. W., Henderson, W. A. Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform. J. Vis. Exp. (117), e54693, doi:10.3791/54693 (2016).

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