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Genetics

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JoVE Journal Genetics
Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
 

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Article DOI: 10.3791/54693-v • 10:37 min • November 30th, 2016
November 30th, 2016 •
1, 1,2, 1, 1, 3, 1
1Digestive Disorders Unit, National Institute of Nursing Research, National Institutes of Health, DHHS, 2National Institutes of Health Research Scholar, Howard Hughes Medical Institute, 3Internal Medicine, Medical School, University of Michigan

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We describe the use of a multiplexed high-throughput gene expression platform that quantitates gene expression by barcoding and counting molecules in biological substrates without the need for amplification. We used the platform to quantitate microRNA (miRNA) expression in whole blood in subjects with and without irritable bowel syndrome.

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Multiplexed High-throughput Gene Expression Platform Circulating MiRNAs Irritable Bowel Syndrome Nucleic Acid Quantification MicroRNA Expression Digestive Disorders Dysregulated Molecular Mechanisms Biomarker Signatures MicroRNA Quantification Amplification-free Automated RNA Extraction Total RNA Purification BM1 Buffer
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