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ויזואליזציה של Assay Resazurin פחם אגר עבור וכמותיות, ספירת בינוני תפוקה של Mycobacteria
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria
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ויזואליזציה של Assay Resazurin פחם אגר עבור וכמותיות, ספירת בינוני תפוקה של Mycobacteria

עברית

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10,135 Views

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09:57 min

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December 14, 2016

DOI:

10.3791/54690-v

DOI:
10.3791/54690-v

•

09:57 min
December 14, 2016

•

3 Views
, , , , , , , ,
1Departments of Microbiology & Immunology,Weill Cornell Medical College, 2Medicine,Weill Cornell Medical College

Summary

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The charcoal agar resazurin assay (CARA) is a semi-quantitative, medium-throughput method to assess activity of test agents against mycobacteria that are replicating, non-replicating, or both. The CARA permits rapid evaluation of time- and concentration-dependent activity and identifies parameters to pursue by colony forming unit (CFU) assays.

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Gold, B., Roberts, J., Ling, Y., Lopez Quezada, L., Glasheen, J., Ballinger, E., Somersan-Karakaya, S., Warrier, T., Nathan, C. Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria. J. Vis. Exp. (118), e54690, doi:10.3791/54690 (2016).

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