A subscription to JoVE is required to view this content. Sign in or start your free trial.
20,641 Views
•
08:08 min
•
October 30, 2016
DOI:
Summary
Automatically generated
Lymphocytes are the major players in adaptive immune responses. Here, we present a lymphocyte purification protocol to determine the physiological functions of the desired molecules in lymphocyte activation in vitro and in vivo. The described experimental procedures are suitable for comparing functional capacities between control and genetically modified lymphocytes.
Read Article
Cite this Article
Lim, J. F., Berger, H., Su, I. Isolation and Activation of Murine Lymphocytes. J. Vis. Exp. (116), e54596, doi:10.3791/54596 (2016).
Copy