Journal
/
Immunology and Infection
/
Misurare Influenza neuraminidasi Inibizione anticorpo I titoli da Enzyme-linked lectina Assay
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay
Please note that all translations are automatically generated. Click here for the English version.

Misurare Influenza neuraminidasi Inibizione anticorpo I titoli da Enzyme-linked lectina Assay

italiano

Automatically Generated

17,847 Views

•

08:11 min

•

September 06, 2016

DOI:

10.3791/54573-v

DOI:
10.3791/54573-v

•

08:11 min
September 06, 2016

•

39 Views
, ,
1Division of Viral Products, CBER,Food and Drug Administration

Summary

Automatically generated

We describe the enzyme-linked lectin assay (ELLA) for measuring influenza neuraminidase (NA)-inhibition antibody titers in sera. The assay uses peanut agglutinin to quantify galactose residues that become accessible when NA removes sialic acid from fetuin-coated, 96-well plates.

Explore More Videos

InfluenzaNeuraminidaseAntibodyELLAEnzyme-linked Lectin AssayH6 Reassortant VirusFetuin-coated PlatePeanut AgglutininHRPVaccine

Read Article

Cite this Article

Gao, J., Couzens, L., Eichelberger, M. C. Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay. J. Vis. Exp. (115), e54573, doi:10.3791/54573 (2016).

Download .ris file

Copy

Share Video

.

Copy