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Die Induktion von Akute Skelettmuskelregeneration durch Cardio Injection
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Developmental Biology
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JoVE Journal Developmental Biology
Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection
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Die Induktion von Akute Skelettmuskelregeneration durch Cardio Injection

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21,369 Views

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07:39 min

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January 01, 2017

DOI:

10.3791/54515-v

DOI:
10.3791/54515-v

•

07:39 min
January 01, 2017

•

12 Views
, , , , ,
1Stem Cell Fate Laboratory,Institute of Genetics and Biophysics “A. Buzzati Traverso”, CNR, 2School of Medicine and Surgery,University of Milano-Bicocca, 3Division of Immunology, Transplantation and Infectious Diseases,San Raffaele Scientific Institute

Summary

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Dieses Manuskript beschreibt ein detailliertes Protokoll akuten Skelettmuskelregeneration in erwachsenen Mäusen und nachfolgende Manipulationen der Muskeln zu induzieren, wie Dissektion, Einfrieren, Schneiden, Routine-Färbung und myofiber Querschnittsfläche Analyse.

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Guardiola, O., Andolfi, G., Tirone, M., Iavarone, F., Brunelli, S., Minchiotti, G. Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection. J. Vis. Exp. (119), e54515, doi:10.3791/54515 (2017).

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