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Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection
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JoVE Journal 発生生物学
Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection

Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection

21,327 Views

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07:39 min

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January 01, 2017

DOI:

10.3791/54515-v

DOI:
10.3791/54515-v

•

07:39 min
January 01, 2017

•

12 Views
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1Stem Cell Fate Laboratory,Institute of Genetics and Biophysics “A. Buzzati Traverso”, CNR, 2School of Medicine and Surgery,University of Milano-Bicocca, 3Division of Immunology, Transplantation and Infectious Diseases,San Raffaele Scientific Institute

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This manuscript describes a detailed protocol to induce acute skeletal muscle regeneration in adult mice and subsequent manipulations of the muscles, such as dissection, freezing, cutting, routine staining, and myofiber cross-sectional area analysis.

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Guardiola, O., Andolfi, G., Tirone, M., Iavarone, F., Brunelli, S., Minchiotti, G. Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection. J. Vis. Exp. (119), e54515, doi:10.3791/54515 (2017).

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