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"Phagosomen Closure Assay" zu visualisieren Phagosomen Formation in drei Dimensionen Mit Total Internal Reflection Fluoreszenz-Mikroskopie (TIRFM)
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Immunology and Infection
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JoVE Journal Immunology and Infection
“Phagosome Closure Assay” to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy (TIRFM)
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"Phagosomen Closure Assay" zu visualisieren Phagosomen Formation in drei Dimensionen Mit Total Internal Reflection Fluoreszenz-Mikroskopie (TIRFM)

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6,328 Views

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10:07 min

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August 26, 2016

DOI:

10.3791/54470-v

DOI:
10.3791/54470-v

•

10:07 min
August 26, 2016

•

2 Views
, , ,
1Inserm U1016, Institut Cochin, CNRS UMR 8104,Université Paris Descartes, Sorbonne Paris Cité

Summary

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We describe an experimental setup to visualize with unprecedented high resolution phagosome formation and closure in three dimensions in living macrophages, using total internal reflection fluorescence microscopy. It allows monitoring of the base of the phagocytic cup, the extending pseudopods, as well as the precise site of phagosome scission.

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Cite this Article

Marie-Anaïs, F., Mazzolini, J., Bourdoncle, P., Niedergang, F. "Phagosome Closure Assay" to Visualize Phagosome Formation in Three Dimensions Using Total Internal Reflection Fluorescent Microscopy (TIRFM). J. Vis. Exp. (114), e54470, doi:10.3791/54470 (2016).

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