Journal
/
/
Voorbereiding en Levering van Protein Microcrystals in lipidische Cubic fase voor Serial Femtoseconde Kristallografie
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

Voorbereiding en Levering van Protein Microcrystals in lipidische Cubic fase voor Serial Femtoseconde Kristallografie

11,073 Views

09:09 min

September 20, 2016

DOI:

09:09 min
September 20, 2016

9 Views
, ,

Summary

Automatically generated

We describe procedures for the preparation and delivery of membrane protein microcrystals in lipidic cubic phase for serial crystallography at X-ray free-electron lasers and synchrotron sources. These protocols can also be applied for incorporation and delivery of soluble protein microcrystals, leading to substantially reduced sample consumption compared to liquid injection.

Read Article