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Voorbereiding en Levering van Protein Microcrystals in lipidische Cubic fase voor Serial Femtoseconde Kristallografie
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
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Voorbereiding en Levering van Protein Microcrystals in lipidische Cubic fase voor Serial Femtoseconde Kristallografie

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11,134 Views

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09:09 min

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September 20, 2016

DOI:

10.3791/54463-v

DOI:
10.3791/54463-v

•

09:09 min
September 20, 2016

•

9 Views
, ,
1The Bridge Institute,University of Southern California, 2Department of Chemistry,University of Southern California, 3School of Molecular Sciences, Center for Applied Structural Discovery at the Biodesign Institute,Arizona State University

Summary

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We describe procedures for the preparation and delivery of membrane protein microcrystals in lipidic cubic phase for serial crystallography at X-ray free-electron lasers and synchrotron sources. These protocols can also be applied for incorporation and delivery of soluble protein microcrystals, leading to substantially reduced sample consumption compared to liquid injection.

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Ishchenko, A., Cherezov, V., Liu, W. Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography. J. Vis. Exp. (115), e54463, doi:10.3791/54463 (2016).

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